Eppendorf Centrifuge 5427 R Manual do Utilizador

Register your instrument! www.eppendorf.com/myeppendorfCentrifuge 5427 ROperating manual

Product descriptionCentrifuge 5427 REnglish (EN)102.4.2 Rotor FA-45-24-11Rotor FA-45-24-11Aerosol-tight fixed-angle rotor for 24 tubesMax. g-force: 25

Product descriptionCentrifuge 5427 REnglish (EN)112.4.3 Rotor FA-45-24-11-KitRotor FA-45-24-11-KitAerosol-tight fixed-angle rotor for 24 tubesMax. g-f

Product descriptionCentrifuge 5427 REnglish (EN)122.4.4 Rotors FA-45-30-11 and F-45-30-11Rotor FA-45-30-11Aerosol-tight fixed-angle rotor for 30 tubes

Product descriptionCentrifuge 5427 REnglish (EN)132.4.5 Rotors FA-45-48-11 and F-45-48-11FA-45-48-11 rotorAerosol-tight fixed-angle rotor for 48 tubes

Product descriptionCentrifuge 5427 REnglish (EN)142.4.6 Rotor F-45-48-5-PCR2.4.7 Rotor S-24-11-ATThis rotor is only intended for use with 1.5 mL/2.0 m

SafetyCentrifuge 5427 REnglish (EN)153 Safety3.1 Intended useThe Centrifuge 5427 R is intended exclusively for indoor use and for separating aqueous

SafetyCentrifuge 5427 REnglish (EN)16Beginning with the first commissioning, the following rotors including the corresponding buckets, carriers and ro

SafetyCentrifuge 5427 REnglish (EN)173.5 Warnings for intended useRead the operating manual and observe the following general safety instructions befo

SafetyCentrifuge 5427 REnglish (EN)183.5.2 Incorrect handling of the centrifuge3.5.3 Incorrect handling of the rotorsCAUTION! Poor safety due to incor

SafetyCentrifuge 5427 REnglish (EN)193.5.4 Extreme strain on the centrifuging tubesCAUTION! Risk of injury from overloaded rotor.The Centrifuge 5427

5409 900.021-02/072013 Copyright © 2013 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior permission of the copy

SafetyCentrifuge 5427 REnglish (EN)203.5.5 Aerosol-tight centrifugation3.6 Safety instructions located on the deviceWARNING! Risk to health due to lim

InstallationCentrifuge 5427 REnglish (EN)214 Installation4.1 Selecting the locationSelect the location for the device according to the following crite

InstallationCentrifuge 5427 REnglish (EN)224.3 Installing the instrumentPrerequisitesThe device is on a suitable lab bench.Perform the following steps

OperationCentrifuge 5427 REnglish (EN)235 Operation5.1 Overview of operating controlsBefore using the Centrifuge 5427 R, familiarize yourself with th

OperationCentrifuge 5427 REnglish (EN)24Abb. 5-2: Display of the Centrifuge 5427 R.Fig. 5-2: Display of the Centrifuge 5427 R.1 Centrifugation time2 K

OperationCentrifuge 5427 REnglish (EN)255.2 Menu navigationProceed as follows to change settings in the device menu:5.3 MenuTab. 5-1: Menu structure o

OperationCentrifuge 5427 REnglish (EN)26The Back menu item can also be found in both menu levels.Back in menu level 2: return to menu level 1.Back in

OperationCentrifuge 5427 REnglish (EN)275.4 Preparing for centrifugation5.4.1 Switching on the centrifuge Switch the centrifuge on using the mains/po

OperationCentrifuge 5427 REnglish (EN)285.4.5 Loading the rotor5.4.5.1 Loading a fixed-angle rotorTo load the rotor, proceed as follows:1. Check the m

OperationCentrifuge 5427 REnglish (EN)295.4.5.2 Loading a swing-bucket rotorPrerequisites• Use a rotor, bucket and adapter combination that is approve

Table of contentsCentrifuge 5427 REnglish (EN)3Operating manual Centrifuge 5427 Rseeon p.Fig.Tab.p.English (EN)Operating manualTable of contents1 Oper

OperationCentrifuge 5427 REnglish (EN)305.4.6 Closing the rotor lid1. Only QuickLock rotor lid: check that the external sealing ring correctly fits in

OperationCentrifuge 5427 REnglish (EN)315.5.3 Temperature monitoringAfter the set temperature has been reached, the centrifuge reacts to temperature d

OperationCentrifuge 5427 REnglish (EN)325.5.5 Continuous coolingContinuous coolingContinuous cooling maintains the rotor chamber at the set temperatur

OperationCentrifuge 5427 REnglish (EN)335.6 CentrifugingBefore using the Centrifuge 5427 R for the first time, familiarize yourself with the operatin

OperationCentrifuge 5427 REnglish (EN)345.6.2 Centrifuging in continuous operationPerform the following steps in the sequence described.1. Use time to

OperationCentrifuge 5427 REnglish (EN)355.6.4 Setting the centrifugation radiusIf an adapter for tubes is used in a rotor, this changes the centrifuga

OperationCentrifuge 5427 REnglish (EN)36

MaintenanceCentrifuge 5427 REnglish (EN)376 Maintenance6.1 Prepare cleaning/disinfection Clean all accessible surfaces of the device and the accessor

MaintenanceCentrifuge 5427 REnglish (EN)386.2.1 Cleaning and disinfecting the device1. Open the lid. Switch off the device with the mains/power switch

MaintenanceCentrifuge 5427 REnglish (EN)394. Rinse the rotors and accessories thoroughly with distilled water. Rinse the rotor bores of fixed-angle ro

Table of contentsCentrifuge 5427 REnglish (EN)45.5 Cooling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

MaintenanceCentrifuge 5427 REnglish (EN)40How to proceed in case of glass breakage1. Remove all splinters and glass powder from the rotor chamber and

TroubleshootingCentrifuge 5427 REnglish (EN)417 TroubleshootingIf you cannot remedy an error with the recommended measures, please contact your local

TroubleshootingCentrifuge 5427 REnglish (EN)427.2 Error messagesProceed as follows if the following error messages appear:1. Remove fault (see remedy)

TroubleshootingCentrifuge 5427 REnglish (EN)43Err 22 Electronics fault.  Switch centrifuge off and back on again after > 20 s.NO E-FAN (ERR 23) El

TroubleshootingCentrifuge 5427 REnglish (EN)447.3 Emergency releaseIf the centrifuge lid cannot be opened, you can activate the emergency release manu

Transport, storage and disposalCentrifuge 5427 REnglish (EN)458 Transport, storage and disposal8.1 Transport Remove the rotor from the centrifuge bef

Transport, storage and disposalCentrifuge 5427 REnglish (EN)46

Technical dataCentrifuge 5427 REnglish (EN)479 Technical data9.1 Power supplyCentrifuge 5427 R9.2 Ambient conditions9.3 Weight/dimensions* The noise l

Technical dataCentrifuge 5427 REnglish (EN)489.4 Application parametersRun time 10 s – 9:59 h, infinite (∞),•10 s – 2 min: can be set in increments

Technical dataCentrifuge 5427 REnglish (EN)499.5 Acceleration times and deceleration times for the Centrifuge 5427 R (according to DIN 58 970)Rotor Ac

Operating instructionsCentrifuge 5427 REnglish (EN)51 Operating instructions1.1 Using this manual Read this operating manual completely before using

Technical dataCentrifuge 5427 REnglish (EN)50

Ordering InformationCentrifuge 5427 REnglish (EN)5110 Ordering Information10.1 Centrifuge 5427 R10.2 Accessories10.2.1 Rotors and rotor lidsOrder no.

Ordering InformationCentrifuge 5427 REnglish (EN)52Aerosol impermeability tested and certified by the Centre of Emergency Preparedness and Response, H

Ordering InformationCentrifuge 5427 REnglish (EN)5310.2.3 Other accessories10.3 SafetyOrder no. (International)Order no. (North America)DescriptionRot

Ordering InformationCentrifuge 5427 REnglish (EN)54

Operating instructionsCentrifuge 5427 REnglish (EN)61.4 Abbreviations usedNNMean sea level (MSL)PCRPolymerase chain reactionRZB/rcfRelative centrifuga

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Product descriptionCentrifuge 5427 REnglish (EN)72 Product description2.1 Main illustrationAbb. 2-1: Front and rear view of the Centrifuge 5427 RFig.

Product descriptionCentrifuge 5427 REnglish (EN)82.2 Delivery package2.3 FeaturesThe high-performance Centrifuge 5427 R has a capacity of 48 × 2 mL

Product descriptionCentrifuge 5427 REnglish (EN)92.4 Rotors2.4.1 Rotor FA-45-12-17Eppendorf centrifuges may only be operated with rotors that are inte