Eppendorf ThermoMixer C Manual do Utilizador

Register your instrument! www.eppendorf.com/myeppendorfEppendorf ThermoMixer® COperating manual

10Product descriptionEppendorf ThermoMixer® CEnglish (EN)2.3 FeaturesYou can use the ThermoMixer C for performing two basic applications of the sample

SafetyEppendorf ThermoMixer® CEnglish (EN)113Safety3.1 Intended useThe ThermoMixer C is intended for use in a molecular biology laboratory.The ThermoM

12SafetyEppendorf ThermoMixer® CEnglish (EN)3.4 Warnings for intended useRead the operating instructions and observe the following general safety info

SafetyEppendorf ThermoMixer® CEnglish (EN)13WARNING! Risk from incorrect supply voltage  Only connect the device to voltage sources which correspond

14SafetyEppendorf ThermoMixer® CEnglish (EN)WARNING! Risk of injury due to incorrect consumables.• Poorly fitting tubes or plates can become detached

SafetyEppendorf ThermoMixer® CEnglish (EN)153.5 Danger symbols on the device NOTICE! Caution! Strong vibration.When mixing at high speeds, items locat

16InstallationEppendorf ThermoMixer® CEnglish (EN)4 Installation4.1 Selecting the locationSelect the device location according to the following criter

OperationEppendorf ThermoMixer® CEnglish (EN)175Operation5.1 Overview of operating controlsAbb. 5-1:Operating controlsThermoMixer CFig. 5-1: Operating

18OperationEppendorf ThermoMixer® CEnglish (EN)Abb. 5-2:Display ThermoMixer CFig. 5-2: Display ThermoMixer C1Program number2Program name3 Program leve

OperationEppendorf ThermoMixer® CEnglish (EN)195.2 Setting the languageThe device is delivered with English as the default language. To set another la

5382 900.016-02/092014Copyright ©2014 Eppendorf AG, Germany. All rights reserved, including graphics and images. No part of this publication may be r

20OperationEppendorf ThermoMixer® CEnglish (EN)When you attach the thermoblock, the device automatically recognizes the mounted thermoblock. The mixin

OperationEppendorf ThermoMixer® CEnglish (EN)215.3.1 Attaching the thermoblock1. First only attach the rear edge of the thermoblock. The writing must

22OperationEppendorf ThermoMixer® CEnglish (EN)5.4 Inserting tubes and plates  Place the suitable thermoblock on the device (see Attaching the the

OperationEppendorf ThermoMixer® CEnglish (EN)23SmartBlock plates, SmartBlock PCR 96 or SmartBlock PCR 384:5.5 Installing the ThermoTopThe ThermoTop is

24OperationEppendorf ThermoMixer® CEnglish (EN)5.6 Temperature controlThe ThermoMixer C can be used for temperature control in a range of 15 °C below

OperationEppendorf ThermoMixer® CEnglish (EN)255.7 Mixing5.7.1 Mixing without temperature control1. In order to switch off the temperature control, u

26OperationEppendorf ThermoMixer® CEnglish (EN)5. After the set mixing time has elapsed, the device stops automatically. • A signal tone sounds.• Th

OperationEppendorf ThermoMixer® CEnglish (EN)275.7.4 Interrupting the mixing processIf you want to add reagents or exchange tubes while mixing, you ca

28OperationEppendorf ThermoMixer® CEnglish (EN)5.8 Menu5.8.1 Navigating in the menuThe menu has 3 levels. To change settings, proceed as follows:5.8.2

OperationEppendorf ThermoMixer® CEnglish (EN)29Go to next higher menu level: BackExit menu item without saving: CancelSave set value: Save• Delete pro

Table of contentsEppendorf ThermoMixer® CEnglish (EN)3Table of contents1 Operating instructions. . . . . . . . . . . . . . . . . . . . . . . . . . . .

30OperationEppendorf ThermoMixer® CEnglish (EN)5.9 ProgramsA program consists of up to four program levels ("steps"). The program levels aut

OperationEppendorf ThermoMixer® CEnglish (EN)314. In order to save the program with the program name, use the menu arrow keys to select Save.5. Select

32OperationEppendorf ThermoMixer® CEnglish (EN)5.9.2 Interval Mix: Creating a program level with pause interval By using the Interval Mix function, yo

OperationEppendorf ThermoMixer® CEnglish (EN)335.9.3 Restricting ramp ratesYou can use the ThermoMixer C to choke the heating rate as well as the cool

34OperationEppendorf ThermoMixer® CEnglish (EN)5.9.5 Loading a saved program5.9.5.1 Loading program prog 1 to prog 5On supply, the prog 1 to prog 5 pr

OperationEppendorf ThermoMixer® CEnglish (EN)355.9.6 Editing programsThere are two ways to change a saved program:• Changing the program via the Edit

36TroubleshootingEppendorf ThermoMixer® CEnglish (EN)6 TroubleshootingIf you cannot remedy an error with the recommended measures, please contact your

TroubleshootingEppendorf ThermoMixer® CEnglish (EN)376.2 Error messagesProblem Cause SolutionThermoblock not recognized• Thermoblock is not compatible

38MaintenanceEppendorf ThermoMixer® CEnglish (EN)7 Maintenance7.1 Setting service intervalsThe ThermoMixer C offers the option of activating a reminde

MaintenanceEppendorf ThermoMixer® CEnglish (EN)39Auxiliary equipment• Lint-free cloth• Mild, soap-based lab cleaner• Dist. waterCleaning the ThermoMix

4Table of contentsEppendorf ThermoMixer® CEnglish (EN)5.8 Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

40MaintenanceEppendorf ThermoMixer® CEnglish (EN)7.3 Disinfection/DecontaminationAuxiliary equipment• Lint-free cloth• Disinfectant.1. Switch the Ther

Transport, storage and disposalEppendorf ThermoMixer® CEnglish (EN)418 Transport, storage and disposal8.1 Transport Use the original packaging for tr

42Technical dataEppendorf ThermoMixer® CEnglish (EN)9 Technical data9.1 Power supply9.2 Weight/dimensions9.3 Ambient conditionsPower connection 100 V

Technical dataEppendorf ThermoMixer® CEnglish (EN)439.4 Application parameters 9.4.1 Temperature control*Heating rate and cooling rate can be throttle

44Technical dataEppendorf ThermoMixer® CEnglish (EN)9.4.2 Mix9.4.3 Time setting9.5 InterfaceThermoblock Mixing frequencycan be set in increments of 50

Ordering informationEppendorf ThermoMixer® CEnglish (EN)4510 Ordering information10.1 Device and accessoriesCAUTION! Poor safety due to incorrect acce

46Ordering informationEppendorf ThermoMixer® CEnglish (EN)10.2 Tubes and platesSmartBlock cryoThermoblock for 24 Cryo tubes5367 000.025 5367000025 1.5

Ordering informationEppendorf ThermoMixer® CEnglish (EN)47All plates are available with different border colors (red, yellow, green and blue) and puri

48Program data formEppendorf ThermoMixer® CEnglish (EN)11 Program data formprogram time temp speed1234567891011121314151617181920

IndexEppendorf ThermoMixer® CEnglish (EN)49IndexAActual temperature... 18Application parameters ...

Table of contentsEppendorf ThermoMixer® CEnglish (EN)511 Program data form. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

IndexEppendorf ThermoMixer® CEnglish (EN)50RRamp rates ... 43Restrict ...

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6Table of contentsEppendorf ThermoMixer® CEnglish (EN)

Operating instructionsEppendorf ThermoMixer® CEnglish (EN)71 Operating instructions1.1 Using this manual Read this operating manual completely before

8Operating instructionsEppendorf ThermoMixer® CEnglish (EN)1.3 Symbols used1.4 Abbreviations usedPCRPolymerase chain reactionrpmRevolutions per minute

Product descriptionEppendorf ThermoMixer® CEnglish (EN)92 Product description2.1 Main illustrationAbb. 2-1:ThermoMixer CFig. 2-1: ThermoMixer C2.2 Del