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HPLC adapter — Instructions for use

Instructions for use

This document supplements the instructions for use of the Centrifuge, but does not replace them.

Therefore, also read the instructions for use. The current version can be found online at

www.eppendorf.com

Product description

The HPLC adapters are designed for centrifugation of HPLC vials with max. 1.5 mL filling volume

in the F-45-18-17-Cryo rotor for Centrifuge 5430/5430 R and Centrifuge 5702/5702 R/5702 RH.

Safety notes

 Prior to using HPLC vials, observe manufacturer specifications on resistance to

centrifugation (max. g-force).

 Do not exceed the maximum tube length of 33 mm (without cap) so the tubes can be

optimally supported by the adapters.

 Do not exceed a g-force of 3,500 x g at a max. load of 9.5 g per rotor bore (adapter + tube +

contents). At a load of up to 8.7 g per rotor bore, the adapters can be centrifuged at max.

g-force (rcf)/rotational speed (rpm).

 Load the rotor symmetrically. When doing so, follow the loading instructions included in the

instructions for use of the centrifuge.

Cleaning and Disinfection

Use a mild cleaning agent for cleaning.

Use alcohol (ethanol, isopropanol) or disinfectants containing alcohol for the disinfection.

Technical data

For the Centrifuge 5430/5430 R, ensure that the g-force displayed during switch-over is

standardized to the corresponding rotor without adapter and, for the Centrifuge 5702/5702 R/

5702 RH, standardized to the A-4-38 rotor. You can achieve the following maximum g-forces (rcf)

at maximum speed (rpm) when adapters are used:

Hint!

The HPLC adapter can be autoclaved (121°C, 20 min).

Rotor, centrifuge Adapters Max. centrifugation

radius (cm)

Max. g-force (rcf)

/ speed (rpm)

F-45-18-17-Cryo,

Centrifuge 5430/5430 R

without adapter

HPLC adapter

9.4

8.3

8,320 / 8,900

7,350 / 8,900

F-45-18-17-Cryo,

Centrifuge 5702/5702 R/5702 RH

without adapter

HPLC adapter

9.4

8,3

2,030 / 4,400

1,800 / 4,400

1 2

Resumo do Conteúdo

Página 1 - Technical data

HPLC adapter — Instructions for use1 Instructions for useThis document supplements the instructions for use of the Centrifuge, but does not replace

Página 2 - Ordering information

HPLC adapter — Instructions for use2 You can calculate the g-force (rcf) for a special adapter using the following formula according to DIN 58 970:r

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