Eppendorf BioSpectrometer basic Manual do Utilizador Página 5
- Página / 104
- Índice
- RESOLUÇÃO DE PROBLEMAS
- MARCADORES
Avaliado. / 5. Com base em avaliações de clientes
5
Table of contents
Eppendorf BioSpectrometer
®
basic
English (EN)
11 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
11.1 Power supply. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
11.2 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
11.3 Weight/dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
11.4 Photometric properties . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
11.5 Further technical parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
11.6 Application parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
12 Evaluation procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
12.1 Absorbance values . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
12.1.1 Blank . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
12.1.2 Background correction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
12.1.3 Cuvette correction. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90
12.2 Evaluation with factor or standard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91
12.3 Evaluation with standard curve/line . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91
12.4 Dilution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92
12.5 Special evaluation procedures for nucleic acids and protein UV . . . . . . . . . . . . . . . . . . . . . . . . 93
12.5.1 Correction A
260
and correction A
280
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
12.5.2 Ratios A260/A280 and A260/A230.. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
12.5.3 Conversion to molar concentrations and nucleic acid quantities . . . . . . . . . . . . . . . . . 94
12.5.4 Calculating the factor for protein in "General Method Parameter" . . . . . . . . . . . . . . . 95
12.6 Special evaluation procedures for the dye methods. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95
12.6.1 Calculating the factor for the dye from the absorbance coefficient . . . . . . . . . . . . . . . 95
12.6.2 Calculation of the FOI . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96
12.6.3 Conversion to amounts of dye. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96
12.7 Dual wavelength . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97
13 Ordering information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99
Certificates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101
- Eppendorf BioSpectrometer 1
- Table of contents 3
- 1 Operating instructions 7
- 1.1 Using this manual 7
- 1.3 Symbols used 8
- 1.4 Abbreviations used 9
- Operating instructions 10
- English (EN) 10
- 2 Product description 11
- 2.1 Main illustration 11
- 2.2 Delivery package 11
- 2.3 Features 12
- 3 Safety 13
- 3.1 Intended use 13
- 3.2 User profile 13
- 3.3 Warnings for intended use 13
- 3.3.2 Damage to device 14
- Do not open the device 16
- 4 Installation 17
- 4.1 Preparing installation 17
- 4.2 Selecting the location 17
- 4.4 Connecting the printer 17
- 4.4.2 Thermal printer DPU-414 18
- Installation 20
- 5 Operation 21
- 5.1.1 Entering text 23
- 5.2 Inserting the cuvette 24
- 5.3.2 Measuring procedure 25
- 5.3.2.1 Selecting a method 25
- 5.3.2.2 Checking parameters 26
- 5.3.2.4 Measuring samples 27
- 5.3.2.5 Finalizing the method 27
- Operation 30
- 6 Methods 31
- 6.1 Selecting a method 31
- 6.2.1 Absorbance method group 32
- 6.2.2 Routine method group 33
- 6.2.3 Basic method group 34
- 6.3 Method parameters 35
- 6.4 Method procedure 40
- 6.4.1 Check parameters 41
- 6.4.2 Measure standards 42
- 6.4.3 Measure samples 43
- 6.4.5 Process results 50
- 6.4.7 Print & export 55
- 7 Functions 59
- 7.1.1 Results memory 60
- Confirm with enter 61
- 7.1.4 Device settings 64
- 7.1.5 Device calibration 64
- 7.1.6 Info 65
- Functions 66
- 8 Maintenance 67
- 8.1 Cleaning 67
- 8.3 Checking the device 69
- Maintenance 70
- 8.4 Replacing fuses 73
- 9 Troubleshooting 75
- 9.1 General errors 75
- 9.2 Error messages 76
- 9.3 Result flags 79
- Troubleshooting 82
- 10.1 Transport 83
- 10.2 Storage 83
- 10.3 Disposal 83
- 11 Technical data 85
- 11.1 Power supply 85
- 11.2 Ambient conditions 85
- 11.3 Weight/dimensions 85
- 11.4 Photometric properties 86
- 11.6 Application parameters 87
- Technical data 88
- 12 Evaluation procedure 89
- 12.1 Absorbance values 89
- 12.1.3 Cuvette correction 90
- 12.4 Dilution 92
- YYYXXXcorrXXX 93
- ACFAA u 93
- S, total 94
- 12.6.2 Calculation of the FOI 96
- 12.7 Dual wavelength 97
- Evaluation procedure 98
- 13 Ordering information 99
- Ordering information 100
- Evaluate your manual 104
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