Eppendorf BioSpectrometer kinetic Manual do Utilizador

nualoSpectrometer® kineticEN)g manual Register your instrument! www.eppendorf.com/myeppendorfEppendorf BioSpectrometer® kineticOperating manual

Product descriptionEppendorf BioSpectrometer® kineticEnglish (EN)102.3 FeaturesThe BioSpectrometer kinetic is a UV/Vis spectrophotometer for measuring

Evaluation procedureEppendorf BioSpectrometer® kineticEnglish (EN)100

101Ordering informationEppendorf BioSpectrometer® kineticEnglish (EN)13 Ordering informationOrder no. (International)Order no. (North America)Descrip

Ordering informationEppendorf BioSpectrometer® kineticEnglish (EN)102

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Evaluate your manualGive us your feedback.www.eppendorf.com/manualfeedbackYour local distributor: www.eppendorf.com/contactEppendorf AG · 22331 Hambur

11SafetyEppendorf BioSpectrometer® kineticEnglish (EN)3Safety3.1 Intended useThe BioSpectrometer kinetic is to be used in molecular biology, biochemis

SafetyEppendorf BioSpectrometer® kineticEnglish (EN)12WARNING! Damage due to UV radiation.Microliter cuvettes, e.g., Hellma® TrayCell (or microliter c

13SafetyEppendorf BioSpectrometer® kineticEnglish (EN)3.3.2 Damage to deviceNOTICE! Damage from the use of aggressive chemicals. Do not use any aggre

SafetyEppendorf BioSpectrometer® kineticEnglish (EN)143.4 Information on product liabilityIn the following cases, the designated protection of the dev

15InstallationEppendorf BioSpectrometer® kineticEnglish (EN)4 Installation4.1 Preparing installation Keep the transport carton and the packing materi

InstallationEppendorf BioSpectrometer® kineticEnglish (EN)164.4.2 Thermal printer DPU-414Connect the thermal printer DPU-414 to the serial printer con

17InstallationEppendorf BioSpectrometer® kineticEnglish (EN)4.5 Connecting PC or USB stick for data exportYou can connect a FAT 32-formatted USB stick

InstallationEppendorf BioSpectrometer® kineticEnglish (EN)18

19OperationEppendorf BioSpectrometer® kineticEnglish (EN)5 Operation5.1 Overview of operating controlsAbb. 5-1: Control panel of the BioSpectrom eter

6136 900.054-03/052014Copyright © 2014 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior permission of the copyr

OperationEppendorf BioSpectrometer® kineticEnglish (EN)20Move the cursor to the left, right, up, down.• Navigation between input fields.• and keys

21OperationEppendorf BioSpectrometer® kineticEnglish (EN)5.1.1 Entering textYou can enter texts when assigning method names and result units. Restrict

OperationEppendorf BioSpectrometer® kineticEnglish (EN)22Prerequisites• The cuvette is free from contamination by dust or fingerprints and free from s

23OperationEppendorf BioSpectrometer® kineticEnglish (EN)5.3.2 Measuring procedure5.3.2.1 Selecting a method5.3.2.2 Checking parameters Use the curso

OperationEppendorf BioSpectrometer® kineticEnglish (EN)245.3.2.3 Measuring the blank and standards5.3.2.4 Measuring samplesFor evaluations without sta

25OperationEppendorf BioSpectrometer® kineticEnglish (EN)5.3.2.5 Finalizing the method5.3.2.6 Optional: process results5.3.2.7 Printing and exporting1

OperationEppendorf BioSpectrometer® kineticEnglish (EN)265.3.3 Important measurement instructions5.3.4 Notes on working with cuvette temperature contr

27OperationEppendorf BioSpectrometer® kineticEnglish (EN)Measured values typical for Eppendorf for temperature control in closed cuvettes, with closed

OperationEppendorf BioSpectrometer® kineticEnglish (EN)28• For efficient temperature control, the volume of the measuring solution in the cuvette shou

29MethodsEppendorf BioSpectrometer® kineticEnglish (EN)6 Methods6.1 Selecting a methodMethods and method templates are delivered preprogrammed. The me

3Table of contentsEppendorf BioSpectrometer® kineticEnglish (EN)Table of contents1 Operating instructions . . . . . . . . . . . . . . . . . . . . . .

MethodsEppendorf BioSpectrometer® kineticEnglish (EN)30You can create new methods in all folders using <New Method>.In Favorites, you can create

31MethodsEppendorf BioSpectrometer® kineticEnglish (EN)6.2.2 Routine method groupThe methods for the Routine group are preprogrammed as fixed methods.

MethodsEppendorf BioSpectrometer® kineticEnglish (EN)32• Correction of the influence of the dye spectrum on the accuracy of the biomolecule measuremen

33MethodsEppendorf BioSpectrometer® kineticEnglish (EN)6.2.4 Advanced method groupDual wavelength• Measurement at two wavelengths and evaluation of th

MethodsEppendorf BioSpectrometer® kineticEnglish (EN)34Unit Selection:mg/mL | μg/mL | ng/mL | pg/mL | μg/μL | mg/dL | μmol/mL | nmol/mL | pmol/mL | p

35MethodsEppendorf BioSpectrometer® kineticEnglish (EN)Factor Value input:Factor.Limit: max. of 6 digits including decimal point.Factor for converting

MethodsEppendorf BioSpectrometer® kineticEnglish (EN)36Correct A260 1 Selection:on | offOnly for the Dye labels method group.Correction of the influen

37MethodsEppendorf BioSpectrometer® kineticEnglish (EN)FOI Selection:none | dye/kb | pmole/μgOnly for the Dye labels method group.Display of the FOI i

MethodsEppendorf BioSpectrometer® kineticEnglish (EN)38Measuring procedureSelection:lin.regr. | endpoint | two pointFor kinetic methods only."Lin

39MethodsEppendorf BioSpectrometer® kineticEnglish (EN)6.4 Method procedureA method procedure is composed of a maximum of 5 steps. The currently activ

Table of contentsEppendorf BioSpectrometer® kineticEnglish (EN)46 Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

MethodsEppendorf BioSpectrometer® kineticEnglish (EN)406.4.1 Check parametersSoftkeys• [Page dn] and [Page up]: Change between 1 to 3 parameter list p

41MethodsEppendorf BioSpectrometer® kineticEnglish (EN)6.4.2 Measure standardsThe first standard to be measured is marked on the display. After the bl

MethodsEppendorf BioSpectrometer® kineticEnglish (EN)426.4.3 Measure samplesThe sample key is used for measuring your samples consecutively. Blank res

43MethodsEppendorf BioSpectrometer® kineticEnglish (EN)Enter dilutionSoftkeys• [Clear dil.]: Delete values for sample dilution.• [OK]: Confirm sample

MethodsEppendorf BioSpectrometer® kineticEnglish (EN)44Result image with dilution and IDResult image with dilution and sample ID

45MethodsEppendorf BioSpectrometer® kineticEnglish (EN)6.4.4 Measure samples: Results displaysThis section contains a display of typical results displ

MethodsEppendorf BioSpectrometer® kineticEnglish (EN)46Multi λ Results display:• Absorbance values at the wavelengthsAdditional data ([Data] softkey):

47MethodsEppendorf BioSpectrometer® kineticEnglish (EN)Proteins direct UVResults display:• Concentration result with absorbance at the measuring wavel

MethodsEppendorf BioSpectrometer® kineticEnglish (EN)48Bacterial densityResults display:• Calculated result with absorbance at the measuring wavelengt

49MethodsEppendorf BioSpectrometer® kineticEnglish (EN)Advanced main groupDual wavelengthResults display:• Concentration result: calculated from Acalc

5Table of contentsEppendorf BioSpectrometer® kineticEnglish (EN)11 Technical data. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

MethodsEppendorf BioSpectrometer® kineticEnglish (EN)506.4.5 Process resultsThe sample measurement is followed by two optional steps in the method seq

51MethodsEppendorf BioSpectrometer® kineticEnglish (EN)6.4.6 Process results: OptionsZoomPress the [Zoom] softkey and select one of the following vers

MethodsEppendorf BioSpectrometer® kineticEnglish (EN)52For all 3 versions, the [reset zoom] softkey will bring you back to the original display of the

53MethodsEppendorf BioSpectrometer® kineticEnglish (EN)More calculationsPress the [More calc.] softkey.Peak detectionPress the [Peaks] softkey. For th

MethodsEppendorf BioSpectrometer® kineticEnglish (EN)54Examples:λ grid: 100 nm, min. Δ Abs: 0.050:The peak is not detected because the λ grid is too l

55MethodsEppendorf BioSpectrometer® kineticEnglish (EN)Linear regressionFor kinetic methods evaluated using the "Linear regression" measurem

MethodsEppendorf BioSpectrometer® kineticEnglish (EN)56Data exportThe data will be transferred as Excel files (.xls) and can be read with Excel versio

57MethodsEppendorf BioSpectrometer® kineticEnglish (EN)6.4.8 Finish the series of measurementsAfter the print & export method step has been finish

MethodsEppendorf BioSpectrometer® kineticEnglish (EN)58

59FunctionsEppendorf BioSpectrometer® kineticEnglish (EN)7Functions7.1 Functions of the User main groupWith the function key or the [Function] softkey

Table of contentsEppendorf BioSpectrometer® kineticEnglish (EN)6

FunctionsEppendorf BioSpectrometer® kineticEnglish (EN)607.1.1 Results memory In the right column, select the method for which you would like to call

61FunctionsEppendorf BioSpectrometer® kineticEnglish (EN)7.1.2 General method parameters In the right column, select the parameter group for which yo

FunctionsEppendorf BioSpectrometer® kineticEnglish (EN)62When programming a method of the Dye labels or Proteins direct UV method groups, you can acce

63FunctionsEppendorf BioSpectrometer® kineticEnglish (EN)7.1.3 Absorbance spectra library7.1.4 Device settings• Specifications for proteins which are

FunctionsEppendorf BioSpectrometer® kineticEnglish (EN)647.1.5 Device calibrationInformation on checking the device is provided separately (see Checki

65MaintenanceEppendorf BioSpectrometer® kineticEnglish (EN)8 Maintenance8.1 Cleaning1. Wipe down the surfaces with a cloth moistened with a mild clean

MaintenanceEppendorf BioSpectrometer® kineticEnglish (EN)663. Clean the cover with a cloth or lint-free cotton swab dampened with a mild cleaning agen

67MaintenanceEppendorf BioSpectrometer® kineticEnglish (EN)8.3.1 Checking the spectrometer unitEppendorf offers a filter kit (BioSpectrometer referenc

MaintenanceEppendorf BioSpectrometer® kineticEnglish (EN)68Abb. 8-1: Inside of the filter box lid (sample)Fig. 8-1: Inside of the filter box lid (samp

69MaintenanceEppendorf BioSpectrometer® kineticEnglish (EN)8.3.1.1 Checking photometric accuracy1. Select the Spectrometer unit function in the Device

7Operating instructionsEppendorf BioSpectrometer® kineticEnglish (EN)1 Operating instructions1.1 Using this manual Read this operating manual complet

MaintenanceEppendorf BioSpectrometer® kineticEnglish (EN)70Have the filter recertified by Eppendorf Service after 2 years.8.3.1.2 Checking wavelength

71MaintenanceEppendorf BioSpectrometer® kineticEnglish (EN)8.3.2 Checking the thermal module• Carry out an inspection at approx. 20 °C. • Make sure th

MaintenanceEppendorf BioSpectrometer® kineticEnglish (EN)728.3.3 Device self-testThe frequency of the automatic self-test (approx. 1 minute) can be se

73MaintenanceEppendorf BioSpectrometer® kineticEnglish (EN)8.5 Decontamination before shipmentIf you are shipping the device to the authorized Technic

MaintenanceEppendorf BioSpectrometer® kineticEnglish (EN)74

75TroubleshootingEppendorf BioSpectrometer® kineticEnglish (EN)9 Troubleshooting9.1 General errorsError Possible cause RemedyMeasuring results are imp

TroubleshootingEppendorf BioSpectrometer® kineticEnglish (EN)769.2 Error messagesYou can exit device displays with error messages using the [OK] softk

77TroubleshootingEppendorf BioSpectrometer® kineticEnglish (EN)Blank measurement: An intensity on a pixel that influences the main, auxiliary or scan

TroubleshootingEppendorf BioSpectrometer® kineticEnglish (EN)78At least two of the entered standard concentrations are identical. Correct the standard

79TroubleshootingEppendorf BioSpectrometer® kineticEnglish (EN)Invalid zoom interval!Error in the process results method step in the Zoom mode.Permiss

Operating instructionsEppendorf BioSpectrometer® kineticEnglish (EN)81.4 Abbreviations usedAAbsorbanceDNADeoxyribonucleic aciddsDNADouble-stranded DNA

TroubleshootingEppendorf BioSpectrometer® kineticEnglish (EN)809.3 Result flagsWarnings and error messages for results are displayed in the bottom rig

81TroubleshootingEppendorf BioSpectrometer® kineticEnglish (EN)The coefficient of determination for the regression evaluation of the standard series i

TroubleshootingEppendorf BioSpectrometer® kineticEnglish (EN)82During the kinetics measurement, the temperature was outside of the allowable range.• A

83Transport, storage and disposalEppendorf BioSpectrometer® kineticEnglish (EN)10 Transport, storage and disposal10.1 Transport Use the original pack

Transport, storage and disposalEppendorf BioSpectrometer® kineticEnglish (EN)84

85Technical dataEppendorf BioSpectrometer® kineticEnglish (EN)11 Technical data11.1 Power supply11.2 Ambient conditionsDo not expose to direct sunligh

Technical dataEppendorf BioSpectrometer® kineticEnglish (EN)8611.4 Photometric properties11.5 IncubationMeasuring principle Single beam absorption spe

87Technical dataEppendorf BioSpectrometer® kineticEnglish (EN)11.6 Further technical parametersCuvette material For measurements in the UV:Quartz glas

Technical dataEppendorf BioSpectrometer® kineticEnglish (EN)8811.7 Application parametersMethods Preprogrammed and freely programmable methods for all

89Evaluation procedureEppendorf BioSpectrometer® kineticEnglish (EN)12 Evaluation procedureThis chapter describes the evaluation procedures available

9Product descriptionEppendorf BioSpectrometer® kineticEnglish (EN)2 Product description2.1 Main illustrationAbb. 2-1: Front and rear viewFig. 2-1: Fro

Evaluation procedureEppendorf BioSpectrometer® kineticEnglish (EN)9012.1.3 Cuvette correctionAll absorbance values which are used for result calculati

91Evaluation procedureEppendorf BioSpectrometer® kineticEnglish (EN)The factor is either entered directly as a parameter during the "Factor"

Evaluation procedureEppendorf BioSpectrometer® kineticEnglish (EN)9212.4 DilutionIn the measure samples method step. entered dilutions are considered

93Evaluation procedureEppendorf BioSpectrometer® kineticEnglish (EN)12.5 Special evaluation procedures for nucleic acids and protein UVThis section co

Evaluation procedureEppendorf BioSpectrometer® kineticEnglish (EN)9412.5.3 Conversion to molar concentrations and nucleic acid quantitiesThe conversio

95Evaluation procedureEppendorf BioSpectrometer® kineticEnglish (EN)12.5.4 Calculating the factor for protein in "General Method Parameter"T

Evaluation procedureEppendorf BioSpectrometer® kineticEnglish (EN)9612.6 Special evaluation procedures for the dye methods12.6.1 Calculating the facto

97Evaluation procedureEppendorf BioSpectrometer® kineticEnglish (EN)12.6.3 Conversion to amounts of dyeThe quantity (dimensions) of dye in the entire

Evaluation procedureEppendorf BioSpectrometer® kineticEnglish (EN)9812.8 KineticsAn absorbance value A or an absorbance difference standardized to a m

99Evaluation procedureEppendorf BioSpectrometer® kineticEnglish (EN)When the kinetic method is evaluated with standards the measurement of a reagent b